The Department of Biotechnology, AJK College of Arts and Science, successfully conducted a one-day workshop on Primer BLAST, designed to equip biotechnology students with essential bioinformatics skills for primer design and validation on 28 February, 2025. The workshop was led by Dr. S. Vijayakumar, Research Scientist, VJ Biotech, who provided an in-depth understanding of Primer BLAST’s applications in molecular biology, genetic research and diagnostics. Students actively participated in hands-on training sessions, where they learned to design highly specific primers for PCR and PCR applications. The workshop covered primer optimization strategies, troubleshooting techniques and real-world case studies, ensuring participants gained practical expertise in computational biology. With enthusiastic participation from students, the event served as a valuable platform for knowledge exchange and skill development. The workshop emphasized the importance of bioinformatics in modern biotechnology, aimed to fostering research-oriented learning and scientific innovation.